• OpenAccess
  • Computer Image Analysis as a Tool for Microbial Viability Assessment: Examples of Use and Prospects  [CBA 2014]
  • DOI: 10.4236/jbm.2014.23001   PP.1 - 6
  • Author(s)
  • Evgeny Puchkov
  • Application of the computer image analysis for improving microbial viability assessment by plate count and fluorescence microscopy was investigated. Yeast cells were used as a model microorganism. The application of the improved methods for the viability assessment of yeast cells after preservation by freezing and freeze-drying was demonstrated.
  • Microbial Viability, Microbial Preservation, Plate Count, Yeast, Computer Image Analysis, Fluorescence Microscopy, Saccharomyces cerevisiae, Cryptococcus terreus, Xanthophyllomyces dendrorhous
  • References
  • [1]
    Davey, H.M. (2011) Life, Death, and in between: Meanings and Methods in Microbiology. Applied and Environmental Microbiology, 77, 5571-5576.
    Barer, M.R. and Harwood, C.R. (1999) Bacterial Viability and Culturability. Advances in Microbial Physiology, 41, 93-137.
    Postgate, J.R. (1969) Viable Counts and Viability. Methods in Microbiology, 1, 611-628.
    Lloyd, D. and Hayes, A.J. (1995) Vigour, Vitality and Viability of Microorganisms. FEMS Microbiology Letters, 133, 1-7.
    Jones, R.P. (1987) Measures of Yeast Death and Deactivation and Their Meaning. Process Biochemistry, 22, 118-128.
    Miles, A. and Misra, S. (1938) The estimation of the bactericidal power of the blood. Journal of Hygiene, 38, 732-748.
    Puchkov, E.O. and Lasukov, Yu.I. (2008) Computer Analyzer KOMPANKOL-M1 to Monitor Microorganism Colonies. Water: Chemistry and Ecology, 5, 29-33. (in Russian)
    Puchkov, E.O. and McCarren, M. (2011) Locating of Nucleic acid Intercalators in Yeast Cells by Image Analysis Combined Fluorescence Microscopy. Journal of Fluorescence, 21, 1009-1013.

Engineering Information Institute is the member of/source content provider to