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Articles
  • OpenAccess
  • Computer Image Analysis as a Tool for Microbial Viability Assessment: Examples of Use and Prospects  [CBA 2014]
  • DOI: 10.4236/jbm.2014.23001   PP.1 - 6
  • Author(s)
  • Evgeny Puchkov
  • ABSTRACT
  • Application of the computer image analysis for improving microbial viability assessment by plate count and fluorescence microscopy was investigated. Yeast cells were used as a model microorganism. The application of the improved methods for the viability assessment of yeast cells after preservation by freezing and freeze-drying was demonstrated.
  • KEYWORDS
  • Microbial Viability, Microbial Preservation, Plate Count, Yeast, Computer Image Analysis, Fluorescence Microscopy, Saccharomyces cerevisiae, Cryptococcus terreus, Xanthophyllomyces dendrorhous
  • References
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    [2]
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    [3]
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    [4]
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    [7]
    Puchkov, E.O. and Lasukov, Yu.I. (2008) Computer Analyzer KOMPANKOL-M1 to Monitor Microorganism Colonies. Water: Chemistry and Ecology, 5, 29-33. (in Russian)
    http://watchemec.ru/en/article/2241/
    [8]
    Puchkov, E.O. and McCarren, M. (2011) Locating of Nucleic acid Intercalators in Yeast Cells by Image Analysis Combined Fluorescence Microscopy. Journal of Fluorescence, 21, 1009-1013.
    http://dx.doi.org/10.1007/s10895-010-0776-0.

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