• OpenAccess
  • Antimutagenic and DNA Damage Protective Activities of a Grape Extract from Vitis vinifera  [GC 2016]
  • DOI: 10.4236/jbm.2016.46001   PP.1 - 8
  • Author(s)
  • Zuzana Rybková, Kateřina Malachová, Jiří Červeň, Hana Sezimová, Petr Pečinka
  • Antimutagenic and DNA protective effect of an extract VinOserae from Vitis vinifera grapes on oxidative DNA damage was investigated. The extract’s ability to inhibit mutagenicity induced by tert-butyl hydroperoxide (t-BHP) and hydrogen peroxide (H2O2) was determined with Ames test using Salmonella typhimurium His? TA102 strain. Inhibition values of 44.2% and 67.0% were detected for t-BHP and H2O2, respectively. A protective ability of the extract against DNA strand scission induced by hydroxyl radicals was studied with plasmid pBluescript II SK(-). The analysis of DNA strand breaks in plasmid DNA showed a significant inhibition of DNA damage.
  • Ames Test, Antimutagenicity, Free Radical Scavenging, DNA Strand Scission, Vitis vinifera
  • References
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