• OpenAccess
  • Screening and Identification of Chitinolytic Actinomycetes and Study on the Inhibitory Activity against Turfgrass Root Rot Disease Fungi  [MIC 2015]
  • DOI: 10.4236/jbm.2015.33009   PP.56 - 65
  • Author(s)
  • Qi Wang, Bihua Duan, Bihua Duan, Rui Yang, Yaorong Zhao, Lu Zhang
  • Objective: This study aimed to separate and pure chitinase producing actinomycetes with stronger activity from lawn soils of Beijing. Method: Morphology and culture characteristics were observed. physiological and biochemical characteristics were detected. 16SrDNA sequence was analyzed. Result: The highest chitinase activity strain was Bn035, whose relative diameter of transparent circle K2 was 3.42 at which the chitinase activity of fermentation broth reached a maximum value of 1.23 U/mL in the 6th day. Crude enzyme solution and bacteria cake of strain Bn035 generated stronger bacterial resistance to all root rot fungi which indicated that the strain had a strong broad-spectrum resistance to root rot fungi. Conclusion: The strain was identified as Streptomyces scabrisporus. It will be a potential biological control function.

  • Strain Bn035, Transparent Circle, Chitinase Activity, Antagonism, 16SrDNA
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