• Voltage-Gated K+ Channel Inhibitor 4-Aminopyridine Inhibits Cell Proliferation and Affects Anti-Proliferative Activities of Cell Cycle-Specific Drugs in Human Breast Cancer Cells   [iCBBE 2015]
  • Author(s)
  • Qin Ru
  • Evidence is growing that potassium channels play important roles in the development and growth of human cancer, including human breast cancer. In this study, we investigated the effect of 4-aminopyridine, an inhibitor of voltage-gated potassium channels, on the growth of human breast cancer MCF-7 cells, with an attempt to know whether pre-arresting tumor cells at a certain phase could affect anti-proliferative activities of cell cycle-specific drugs, and found that 4-aminopyridine significantly inhibited the cell proliferation and induced apoptosis in a dose-dependent manner. 4-aminopyridine also significantly blocked the cell cycle in G1 phase at 1 mmol/l, and induced mainly S phase arrest at 5 and 10 mmol/l. Further studies exhibited strong synergistic action between 4-aminopyridine (5 mmol/l) and S phase-specific drug 5-FU, and antagonistic action between 4-aminopyridine (5 mmol/l) and G2/M phase- specific drug paclitaxel, indicating that cell cycle parameters should be considered in the combination therapy involving cell cycle-specific agents.
  • Voltage-Gated K+ Channel, 4-Aminopyridine, Anti-Proliferative Activities, Cell Cycle-Specific Drugs, Human Breast Cancer Cells
  • References

Engineering Information Institute is the member of/source content provider to