Detection of C-reactive protein based on a Dynabeads-labeled sandwich immunoassay by using a GMI biosensor

Articles

Detection of C-reactive protein based on a Dynabeads-labeled sandwich immunoassay by using a GMI biosensor [iCBBE 2015]
Author(s)
Zhen Yang
ABSTRACT
A Dynabead-labeled sandwich immunoassay had been developed using a giant magnetoim-pedance (GMI) biosensor for the detection of C-reactive protein (CRP). The biosensor involv-ing the sensing elements of Cr/Cu/NiFe/Cu/NiFe was fabricated by Micro Electro-Mechanical system (MEMS) technology. The immune reaction of biomarkers CRP was accomplished on a separated Au film substrate surface with a self-assembled layer. The fundamental principle for detection of CRP based on GMI biosensor was that Dynabeads were employed as magnetic la-bels of CRP, and CRP can be monitored by detecting the fringe field of Dynabeads using magnetic sensing elements. We observed that the GMI ratio were significantly enhanced due to the presence of CRP combined with Dynabeads. The rise decreased as the CRP concentra-tion increased. A lower detectable concentration of 10 ng/ml was achieved in present work. The GMI-based biosensor provides a new method to rapid and sensitive detection CRP, which has a large potential for bio-application.
KEYWORDS
Detection of C-reactive protein, Dynabeads-labeled sandwich immunoassay, GMI biosensor
References