Effects of the
pretreatment with captopril on myocardial apoptosis in myocardial injury and
gp130 expression were studied in this experiment. 66 Male Wistar rats were
randomly divided into control, Iso-induced injury and Cap-pretreated groups. The
myocardial cell apoptosis was examined by TUNEL method, the expression of Bcl-2
and Bax proteins was detected by immunohistochemistry, and the expression of
gp130 in the myocardial tissue was measured by Western blot. Compared with that
in Iso-induced injury group, the apoptotic index of rats in Cap-pretreated
group decreased, the level of Bax and gp130 expression decreased, but the
expression of Bcl-2 increased (P <0.01 or < 0.05).
Captopril could interfere with the apoptosis by affecting the regulation of
apoptosis-related genes and the expression of gp130 in the process of
myocardial tissue injury. Captopril may exert its inhibitory effect on the
apoptosis both directly by blocking the formation of AngⅡ and
indirectly by inhibiting the expression their receptor gpl30 in the myocardial
injury.
