• OpenAccess
  • The Structural Proteins of Bdellovibrio bacteriovorus Bacteriophage MAC-1  [ICAH 2016]
  • DOI: 10.4236/wjet.2016.43B002   PP.7 - 13
  • Author(s)
  • Rajinder S. Ranu, Renee Gallegos, Mary Althauser, Lisa Wolfe
  • In the present investigation the structural proteins associated with MAC-1 bacteriophage have been characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE); tandem mass spectrometry of protein bands from SDS-PAGE gel; from the open reading frames (ORFs) deduced from MAC-1 genome sequence and amino acid sequence homology searches from the Uniprot database (up000002418). Results have led to the identification of at least three structural proteins associated with MAC-1 phage genome. They are: capsid protein (~55,000-daltons); spike protein (~22,000-daltons) and a low molecular weight DNA binding protein (~4000-dal- tons). In addition, two other minor proteins were tentatively identified as replicative and scaffold proteins based on two to three unique peptides from mass spectrometry data. However, other proteins coded (ORFs) by phage genome remain to be identified.

  • Bdellovibrio bacteriovorus Bacteriophage, MAC-1 Phage Structural Proteins, MAC-1 Genome, MAC-1 Genes (ORFs)
  • References
  • [1]
    Stolp, H. and Starr, M.P. (1963) Bdellovibrio bacteriovorus gen. et sp. n., a Predatory, Ectoparasitic and Bacteriolytic Microorganism. Antonie van Leeuwenhoek J. Microbiol, 99, 217-248.
    Seidler, R.J. and Starr, M.P. (1969) Isolation and Characterization of Host-Independent Bdellovibrios. J. Bacteriol, 100, 769-785.
    Rittenberg, S.C. (1983) Bdellovibrio: Attack, Pene-tration and Growth on Its Prey. American Society of Microbiology News, 49, 435-439.
    Ruby, E.D. (1992) The Genus Bdellovibrio. In: Balows, A., Triiper, H.G., Dworkin, M., Harder, W. and Heinz- Schleifer, K., Eds., The Pro-karyotes, Volume IV, Springer, New York, 3400-3415.
    Rendulic, S., Jagtap, P., Rosinus, A., Eppinger, M., Baar, C., Lanz, C., Keller, H., Lambert, C., Evans, K.J., Goesmann, A., Meyer, F., Sockett, R.E. and Schuster, S.C. (2004) A Predator Unmasked: Life Cycle of Bdellovibrio bacteriovorus from a Genome Perspective. Science, 303, 689-692.
    Harini, K., Ajila, V. and Hegde, S. (2013) Bdellovibrio bacteriovorus: A Future Antimic, Vrobial Agent? J Indian Soc Periodonto, 17, 823-825.
    Olanya, O.M. and Lakshman, D.K. (2015) Potential of Predatory Bacteria as Biocontrol Agents for Foodbourne and Plant Pathens. J. Plant Pathology, 97, 405-417
    Diedrich, D.L., Denny, C.F., Hashimoto, T. and Conti, S.F. (1970) Facultatively Parasitic Strain of Bdellovibrio bacteriovorus. J. Bac-teriol, 101, 989-996.
    Hashimoto, T., Diedrich, D.L. and Conti, S.F. (1970) Isolation of Bacteriophage for Bdel-lovibrio bacteriovorus. J. Virol, 5, 97-98.
    Althauser, M., Samsonoff, W.A., Anderson, C. and Canti, S.F. (1972) Isolation and Preliminary Characterization of Bacteriophages of Bdellovibrio bacteriovorus. J. Virol, 10, 516-523.
    Varon, M. and Levisohn, R. (1972) Three-Membered Parasitic System: A Bacteriophage, Bdellovibrio bacteriovorus, and Escherichia coli. J. Virol, 9, 519-525.
    Roberts, R.C., Keefer, M.A. and Ranu, R.S. (1987) Characterization of Bdellovibrio bacteriovorus Bacteriophage MAC- 1. J.Gen. Microbiol, 133, 3065-3070.
    Roberts, R.C. and Ranu, R.S. (1987) Transfection of Bdellovib-rio bacteriovorus with bacteriophage MAC-1 DNA. FEMS Microbiol let, 43, 207-211.
    Ranu, R.S. (2002) Natural Single Stranded DNA. In: En-cyclopedia of life Sciences, John Wiley & Sons, 1-8.
    Adams, M. (1959) Bacteriophages. Wiley Interscience, New York.
    Hershey, A.D., Kalmansogn, D. and Bronfenbrenner, J. (1943) Quantitative Methods in the Study of the Phage-Anti- serum Reaction. Journal of Immunology, 46, 267-280.
    Ranu, R.S., Gowda, S., Scholthof, H., Wu, F.G. and Shepherd, R.J. (1996) In Vitro Translation of the Full-Length RNA Transcript of Figwort Mosaic Virus (Cau-limovirus). Gene Expr, 5, 143-145.
    Maniatis, T., Fritsch E.F. and Sambrook, J.K. (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor.
    Roberts, R.C. (1987) Characterization of Bdellovibrio bacteriovorus Bacteriophage MAC-1. M.S. Thesis, Submitted to Colorado State University, Fort Col-lins.
    Gallegos, R. (1988) Studies on the Genome of Bacteriophage MAC-1. M. S. Thesis, Submitted to Colorado State University, Fort Collins
    Ranu, R.S. (1995) DNA Sequencing by delta-Taq DNA Polymerase. BioTechniques, 18, 390-393.
    Sanger, F., Milklen, S. and Coulson, A.R. (1977) DNA Sequencing with Chain-Terminating Inhibitors. Proc. Natl. Acad. Sci. USA, 74, 5463-5467.
    Fan, J., Ranu, R.S., Smith, C., Ruan, C. and Fuller, C.W. (1996) DNA Sequencing with [Alpha-33P]-Labeled ddNTP Terminators: A New Approach to DNA Sequencing with ThermoSequenase? DNA Polymerase. BioTechniques, 21, 1132-1137.
    Laemmeli, U.K. (1970) Cleavage of Structural Proteins during the Assembly of the Head of Bacteri-ophage T4. Nature (London), 227, 680-685.
    Saveliev, S.V., Woodroofe, C.C., Sabat, G., Adams, C.M. and Klaubert, D. (2013) Mass Spectrometry Compatible Surfactant for Optimized In-Gel Protein Digestion. Anal. Chem, 907-914.
    Keller, A., Nesvizhskii, A.I., Kolker, E. and Aebersold, R. (2002) Empirical Statistical Model to Estimate the Accuracy of Peptide Identifications Made by MS/MS and Database Search. Analytical Chemistry, 74, 5383-5392.
    Searle, B.C., Tuner, M. and Nesvizhskii, A.I. (2008) Improving Sensitivity by Probabilistically Combining Results from Multiple MS/MS Search Methodologies. J. Proteome Research, 7, 245-253.
    Kall, L., Storey, J.D., MacCoss, M.J. and Noble, W.S. (2008) Assigning Significance to Pepides Identified by Tandem Mass Spectrometry Using Decoy Database. J. Proteome Research, 7, 29-34.
    Nesvizhskii, A.I., Keller, E. and Aebersold, R. (2003) A Statistical Model for Identifying Proteins by Tandem Mass Spectrometry. Analytical Chemistry, 75, 4646-4658.
    Godson, G.N. (1978) The Isometric Phages. In: Denhardt, D.T., Dressler, D.and Ray, D.S., Eds., The Single Stranded DNA Phages, Cold Spring Harbor Laboratory, Cold Springer Harbor, 103-112.
    Brentlinger, K.L., Hafenstein, S., Novak, C.R., Fane, B.A., Borgon, R., McKenna, R. and Agband-je-McKenna, M. (2002) Microviridae, a Family Divided: Isolation, Characterization, and Genome Sequence of phiMH2K, a Bacteriophage of the Obligate Intracellular Parasitic Bacterium Bdelloovibrio bacteriovorus. J. Bacteriology, 184, 1089-1094.

Engineering Information Institute is the member of/source content provider to