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Articles
  • OpenAccess
  • Amino Acid Requirements of Gilthead Bream (Sparus aurata) Juveniles  [NFS 2016]
  • DOI: 10.4236/wjet.2016.43B004   PP.18 - 24
  • Author(s)
  • M. M. Gaber, M. El-S. Salem, M. A. Zaki, A. M. Nour
  • ABSTRACT
  • In one trial 360 gilthead bream fingerlings received two different feed mixtures containing two different levels of protein (diet A 400 and B 360 g?kg?1). The average initial live weight was about 2.2 ± 0.1 g fish?1 in each replicate of all treatments. Sixty fish per tank were stocked randomly in 6 fiberglass tanks (1 m3 each). Fish were fed with experimental diets at rate 8% of the body weight daily. The Amino Acids (AA) content was analyzed in the diets, whole fish and muscle at the end of the growth period (60 days). The results indicated that at the end of experiment gilthead bream fingerlings had reached an average live weight 18.5 and 16.2 g fish?1 for fish fed diet A and B respectively. Also the increase of protein levels in the feed to 40% caused a significant increase in the content of Indispensable Amino Acid (IAA) and dispensable amino acid (as %v of whole fish and muscle of wet weight). Two methods were subsequently used to estimate the quantitative IAA requirements of gilthead bream fry based on the hypothesis that (a) the dietary requirement pattern of IAA reflected the tissue pattern and (b) the rate of daily deposition in the fish could be equated with the dietary requirement as percentage of 100 g?1 diet. The quantitative IAA requirement (%) of gilthead bream (Sparus aurata) diets was as follow: Lysine 2.27, Methionine 1.09, Therionine 0.61, Leucine 2.4, Isoleucine 0.47, Histidine1.6, Arginine 1.39, Phenylalanine 1.43, Valine 1.22 and Tryptophane 0.42.
  • KEYWORDS
  • Amino Acid Requirements, Gilthead Bream, Whole Body, Tissues
  • References
  • [1]
    Peres, H. and Oliva-Teles, A. (1999) Influence of Temperature on Protein Utilization in Juvenile European Seabass (Dicentrarchus labrax). Aquaculture, 170, 337-348.
    http://dx.doi.org/10.1016/S0044-8486(98)00422-0
    [2]
    Ogino, C. and Nanri, M. (1980) Relationship between the Nutritive Value of Dietary Protein for Rainbow Trout and the Essential Amino Acid Composition. Bulletin Japanese Science Society Fish, 146, 109-112.
    http://dx.doi.org/10.2331/suisan.46.109
    [3]
    Cowey, C.B. and Tacon, A.G.J. (1981) Fish Nutrition Relevance to Invertebrates. Presented at 2nd Int. Cont. on Aquaculture Nutrition Biochemical Approaches to Shell Fish Nutrition, Univ. of Delaware, 27-29 October 1981.
    [4]
    Wilson, R.P. and Poe, W.E. (1985). Apparent Digestible Protein and Energy Coefficients of Common Feed Ingredients for Channel Catfish. Progressive Fish-Culturist, 47, 154-158.
    http://dx.doi.org/10.1577/1548-8640(1985)47<154:ADPAEC>2.0.CO;2
    [5]
    Tibaldi, E. and Kaushik, S.J. (2005) Amino Acid Requirements of Mediterranean Fishes Pecies. In: Montero, D., Basurco B., Nengas, I., Alexis, M. and Izquierdo, M., Eds., Mediterranean fish nutrition Zaragoza: CIHEAM Cahiers Options Méditerranéennes, n. 63
    [6]
    Fuller, M.F., Ston, R.M., Baird, B.A. and Atkeinson, T. (1979) The Optimal Amino Acid Supplementation. British Journal of Nutrition, 41, 321-331.
    http://dx.doi.org/10.1079/BJN19790041
    [7]
    Mambrini, M. and Kaushik, S.J. (1995) Indispensable Amino Acid Requirements of Fish: Correspondence between Quantitative Data and Amino Acid Profiles of Tissue Proteins. J. Appl. Ichthyol, 11, 240-247.
    http://dx.doi.org/10.1111/j.1439-0426.1995.tb00024.x
    [8]
    Wilson, R.P. and Cowey, C.B. (1985) Amino Acid Composition of Whole Body Tissue of Rainbow Trout and Atlantic Salmon. Aquaculture, 48, 373-376.
    http://dx.doi.org/10.1016/0044-8486(85)90140-1
    [9]
    Golterman, H.L., Clymo, R.S. and Ohnstad, M. A. M. (1978) Methods of Physical and Chemical Analysis of Fresh Waters. Blackwell Scientific Publications, Oxford, 214 p.
    [10]
    AOAC (Association of Official Analytical Chemists) (2006) Official Methods of Analysis. 18th Edition, Washington DC, 1018.
    [11]
    Mason, E.A., Lin, S.L. and Gatland, I.R. (1079) Mobility and Diffusion of Protons and Deuterons in Helium—A Runaway Effect. Journal of Physics B: Atomic and Molecular Physics.
    [12]
    Blauth, O.J., Chareinski, M., Berlie, H. (1963) A new Rapid Method for Determining Tryptophan. Anal. Biochem, 6, 69.
    http://dx.doi.org/10.1016/0003-2697(63)90009-5
    [13]
    Tibaldi, E. and Lanari, D. (1991) Optimal Dietary Lysine Levels for Growth and Protein Utilisation of Fingerling Sea Bass (Dicentrarchus labrax) Fed Semipurified Diets. Aquaculture, 95, 297-304.
    http://dx.doi.org/10.1016/0044-8486(91)90095-O
    [14]
    Ogino, C. (1980) Requirements of Carp and Rainbow Trout for Essential Amino Acid. Bulletin Japanese Science Society Fish, 46, 171-174.
    [15]
    Schwarz, F.J., Zeitler, M.H. and Kirchgessner, M. (1984) Ansatz and Vewertung von protein beikarpfen (Cyprinus carpio L) mit untersch-iedlicher protein und Energiev-sorgung. Arch. Tierernbaha, 34, 165-178.
    http://dx.doi.org/10.1080/17450398409426940
    [16]
    Zeitler, M.H., Kirchgessner, M. and Schwarz, F.J. (1984) Effects of Different Protein and Energy Supplies on Carcass Composition of Carp (Cyprinus carpio L). Aquaculture, 36, 37-48.
    http://dx.doi.org/10.1016/0044-8486(84)90052-8
    [17]
    D’Mello, J.P.F., Ed. (1994) Amino Acids in Farm Animal Nutrition. CAB International, Wallingford.
    [18]
    Schwarz, F.J. and Kirchgessner, M. (1988) Amino Acid Composition of Carp (Cyprinus carpo L) with Varying Protein and Energy Supplies. Aquaculture, 72, 307-317.
    http://dx.doi.org/10.1016/0044-8486(88)90219-0
    [19]
    Wunsche, J. and Steffens, W. (1968) Der Gehalt an essentiellen Aminosauren im protein von parpfen (Cyprinus carpio). Regeabogenforelle (Salmo gairdoeri). Kleiner Marane (Corgenus albule). Hecht Esor Lucius und Aal (Anguilla Anguilla). Z. Fisherei NF, 16, 301-304.
    [20]
    Gaber, M.M., Zaki, M.A., Nour, A.M. and Salem, M.El.-S. (2015) Amino Acid Requirements of Sea Bass (Dicentrar-chus labrax). International Journal of Agriculture Science and Natural Resources, 2, 40-45.
    [21]
    Gaber, M.M. (1994) Amino Acid Requirements and Body Composition of Tilapia Mossambicus. Annals of Agriculture Science of Moshtohor, 33, 1225-1236.
    [22]
    Njaa L.R. and Utne, F. (1982). A Comparison of the Amino Acid Compositions of Fifteen Species of Whole Fish. Fisk. Skr., Ernearing, 2, 25-33.
    [23]
    Gunasekera, R.M., Shim, K.F. and Lam, T.J. (1997) Influence of Dietary Protein Content on the Distribution of Amino Acids in Oocytes, Serum and Muscle of Nile Tilapia (Oreochromis niloticus). Aquaculture, 152, 205-221.
    http://dx.doi.org/10.1016/S0044-8486(96)01526-8
    [24]
    Gatlin, D.M. (1987) Whole Body Amino Acid Composition and Comparative Aspects of Amino Acid Nutrition of Goldfish, Golden Shiner and Flathead Minnow. Aquaculture, 60, 223-229.
    http://dx.doi.org/10.1016/0044-8486(87)90289-4
    [25]
    El-Saidy, D.M. and Gaber, M.M. (1998) Amino Acid Requirements and Body Composition of Nile Tilapia (Oreochromis niloticus). Annals of Agriculture Science of Moshtohor, 36, 163-174.
    [26]
    Steinhart, H. (1983) Moglich keiten der Aufwertun von Elwessfutter mitteln in der schweinemast. Kraftfutter, 66, 314-318.
    [27]
    Lovell, R.T. (1989) Nutrition and Feeding of Fish. Van Nostrand Reinhold, New York.
    http://dx.doi.org/10.1007/978-1-4757-1174-5
    [28]
    Jauncey, K., Tacon, A.C.J. and Jackson, A.J. (1983) The Quantitative Essential Amino Acid Requirements of Oreochromis (Sarotherodon) Mossambicus. In: Fishelson, J. and Yaron, Z., Eds., Proc. 1st lntl. Symp. on Tilapia in Aquaculture, Tel Aviv University, Tel Aviv, 328-337.
    [29]
    Wilson, R.P. and Halver, J.E. (1968) Protein and Amino Acid Requirements of Fishes. AnnU. Rev. Nutr, 6, 225-244.
    http://dx.doi.org/10.1146/annurev.nu.06.070186.001301
    [30]
    Zeitler, M.H., Schwarz, F.J. and Kirchgessner, M. (1983) Wachstum und Nabrstoffaufwand bei karpfen (Cyprinus carpio L) mit unterschiedlicher protein-und Energieversorgugn I. Mitteilung: Versuchsplan, Rationszusammensetzung. Nahrstoffreedaulichkei Z. Tierphysiol Tierernahr Futtermittelkd, 49, 86-87.
    [31]
    Tibaldi, E., Tulli, F. and Corsin, F. (1996) Current Estimates of the Essential Amino Acid Requirements of Sea Bass (D. labrax) as Determined by Unconventional Methods. In: Chatain, B., Saroglia, M., Sweetman, J. and Lavens, P., Eds., Proceedings of the International Workshop on Seabass and Seabream Culture: Problems and Prospects, Verona, European Aquaculture Society, Oostende, 320-323.
    [32]
    Boorman, K.N. (1980) Dietary Constraints on Nitrogen Retention. In: Batter, P.J. and Lindsey, D.B., Eds., Protein Deposition in Animals, Butterworths, London, 147-166.
    http://dx.doi.org/10.1016/b978-0-408-10676-4.50013-6
    [33]
    Thebault, H., Alliot, E. and Pastoureaud, A. (1985) Quantitative Methionine Requirement of Juvenile Sea-Bass (Dicentrarchus labrax). Aquaculture, 50, 75-87.
    http://dx.doi.org/10.1016/0044-8486(85)90154-1

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