• OpenAccess
  • Expression of Genes Affecting Skin Coloration and Sugar Accumulation in Apple Fruits at Ripening Stages in High Temperatures  [ICAH 2015]
  • DOI: 10.4236/wjet.2015.33B002   PP.7 - 12
  • Author(s)
  • Seon Ae Kim, Soon Young Ahn, Hyun Hee Han, In Chang Son, Hae Keun Yun
  • High temperature is one of the most important environmental factors that affect the successful cultivation of apple (Malus × domestica Borkh). The expression of genes related with anthocyanin synthesis and sugar accumulation by high temperature treatment was investigated in the fruits of “Fuji” apple at different developmental stages in different temperature conditions through real- time PCR. In the initial ripening stages in fruits, there was high expression of genes associated with fruit ripening, flavonoid compound accumulation, and coloration in high temperatures. Expression of phenylalanine ammonia-lyase, chalcone synthase, flavone 3-hydrogenase, and malate dehydrogenase genes increased gradually in initial ripening stages, while sharply reduced at 24 hr after treatment. In addition, there was no significant difference in the expression of all temperature treatments in late ripening stages. In the further work, investigation of expression levels of various genes could be conducted in the level of transcriptomes from fruits at the early stages to get meaningful information of ripening metabolism in apples in high temperatures.

  • Anthocyanin, Apple, High Temperature, Skin Coloration, Sugar Content
  • References
  • [1]
    King, G.J. (1995) Progress of Apple Genetic Mapping in Europe. HortScience, 30, 749-749.
    Saure, M.C. (1990) External Control of Anthocyanin Formation in Apple. Scientia Horticulturae, 42, 181-218.
    Tomana, T. and Yamada, H. (1988) Change in Sugar Composition during Maturation Stage of Apple Fruit Grown at Different Locations. Journal of the Japanese Society for Horticultural Science, 57, 178-183.
    Ubi, B.E., Honda, C., Bessho, H., Kondo, S., Wada, M., Kobayashi, S. and Moriguchi, T. (2006) Expression Analysis of Anthocyanin Biosynthetic Genes in Apple Skin: Effect of UV-B and Temperature. Plant Science, 170, 571-578.
    Mori, K., Goto-Yamamoto, N., Kitayama, M. and Hashizume, K. (2007) Loss of Anthocyanins in Red-Wine Grape under high Temperatures. Journal of Experimental Botany, 58, 1935-1945.
    Lo Piero, A.R., Puglisi, I., Rapisarda, P. and Petrone, G. (2005) Anthocyanins Accumulation And related Gene Expression in Red Orange Fruit Induced by Low Temperature Storage. Journal of Agricultural and Food Chemistry, 53, 9083-9088.
    Ban, Y., Kondo, S., Ubi, B.E., Honda, C., Bessho, H. and Moriguchi, T. (2009) UDP-Sugar Biosynthetic Pathway: Contribution to Cyaniding 3-Galactoside Biosynthesis in Apple Skin. Planta, 230, 871-881.
    Steyn, W., Holcroft, D., Wand, S. and Jacobs, G. (2005) Red Co-lour Development and Loss in Pears. Acta Horticulturae, 671, 79-85.
    Honda, C., Kotoda, N., Wada, M., Kondo, S., Kobayashi, S., Soejima, J., Zhang, Z., Tsuda, T. and Moriguchi, T. (2002) Anthocyanin Biosynthetic Genes Are Coordinately Expressed during Red Coloration in Apple Skin. Plant Physiology and Biochemistry, 40, 955-962.
    Lancaster, J.E. (1992) Regulation of Skin Color in Apples. Critical Reviews in Plant Sciences, 10, 487-502.
    Lee, J.C., Tomana, T., Naoki, U. and Ikuo, K. (1979) Physiological Study on the Anthocyanin Development in Grape-I. Effect of Fruit Temperature on the Anthocyanin Development in “Kyoho” Grape. Korean Journal of Horticultural Scicence and Technology, 20, 55-65.
    Kondo, S., Hiraoka, K., Kobayashi, S., Honda, C. and Terahara, N. (2002) Changes in the Expression of Anthocyanin Biosynthetic Genes during Apple Development. Journal of the American Society for Horticultural Science, 127, 971-976.
    Hulme, M., Zhao, Z.C. and Jiang, T. (1994) Recent and Future Climate Change in East Asia. International Journal of Climatology, 14, 637-658.
    Chang, S., Puryear, J. and Cairney, J. (1993) A Simple and Efficient Method for Isolating RNA from Pine Trees. Plant Molecular Biology Reporter, 11, 113-116.
    Lakso, A.N. (1994) Apple. Handbook of Environmental Physiology of Fruit Crops, 1, 3-42.
    Yoon, C.G., Son, I.C. and Kim, D.I. (2010) Effects of Temperature during Growth Stage on Tree Growth and Fruit Quality in “Fuji”/M.9 Apples. Journal of the Korean Society of International Agriculture, 22, 143-147.
    Beruter, J. (1985) Sugar Accumulation and Changes in the Activities of Related Enzymes during Development of the Apple Fruit. Journal of Plant Physiology, 121, 331-341.
    Atkinson, R.G., Schroder, R., Hallett, I.C., Cohen, D. and MacRae, E.A. (2002) Overexpression of Polygalacturonase in Transgenic Apple Trees Leads to a Range of Novel Phenotypes Involving Changes in Cell Adhesion. Plant Physiology, 129, 122-133.
    Costa, F., P. Peace, C., Stella, S., Serra, S., Musacchi, S., Bazzani, M., Sansavini, S. and Van de Weg, W.E. (2002) QTL Dynamics for Fruit Firmness and Softening around an Ethylene-Dependent Polygalacturonase Gene in Apple Malus × domestica Borkh.). Journal of Experimental Botany, 61, 3029-3039.
    Boss, P.K., Davies, C. and Robinson, S.P. (1996) Analysis of the Expression of Anthocyanin Pathway Genes in Developing Vitis vinifera L. cv Shiraz Grape Berries and the Implications for Pathway Regulation. Plant Physiology, 111, 1059-1066.
    Noro, S., Ichinohe, H. and Obra, N. (1991) Effects of Controlled Air Temperature on Development of Anthocyanin, Sugar and Citramalic Acid during Maturation of the Apple Cultivar Starking Delicious. Bulletin of the Aomori Apple Experiment Station, 27, 111-123.
    Lee, H.C. (1999) Physiologycal and Ecological Factors Affecting Fruit Coloration and Color Enhancement in Malusdomestica Borkh. cv. “Fuji”. Ph. D. Thesis, Seoul National University, Seoul.
    Lister, C.E., Walker, J.E. and Lancaster, J.R.L. (1996) Developmental Changes in En-zymes of Flavonoid Biosynthesis in the Skins of Red and Green Apple Cultivars. Journal of the Science of Food and Agriculture, 71, 313-320.<313::AID-JSFA586>3.0.CO;2-N
    Ju, Z.G., Liu, C.L. and Yuan, Y.B. (1995) Activities of Chalcone Synthase and UDPGal: Flavonoid 3-O-glycosyl- transferase in Relation to Anthocyanin Synthesis in Apple. Scientia Horticulturae, 63, 175-185.
    Ju, Z.G., Yuan, Y.B., Liou, C.L. and Xin, S.H. (1995) Relationships among Phenylalanine Ammonia-Lyase Activity, Simple Phenol Concentrations and Anthocyanin Accumulation in Apple. Scientia Horticulturae, 61, 215-226.
    Luedeling, E., Girvetz, E.H., Semenov, M.A. and Brown, P.H. (2011) Climate Change Affects Winter Chill for Temperate Fruit and Nut Trees. PLoS ONE, 6, e20155.
    Sugiura, S. and Yokozawa, M. (2004) Impact of Global Warming on Environment for Apple and Satsuma mandarin Production Estimated from Change of the Annual Mean Temperature. Journal of the Japanese Society for Horticultural Science, 73, 72-78.
    Bergqvist, J., Dokoozlian, N. and Ebisuda, N. (2001) Sunlight Exposure and Temperature Effects on Berry Growth and Composition of Cabernet Sauvignon and Grenache in the Central San Joaquin Valley of California. American Journal of Enology and Viticulture, 52, 1-7.
    Azuma, A., Yakushiji, H., Koshita, Y. and Kobayashi, S. (2012) Flavonoid Biosynthesis-Related Genes in Grape Skin Are Differentially Regulated by Temperature and Light Conditions. Planta, 236, 1067-1080.

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